Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Specificity
Binds an internal epitope of human MRP5. Other species not tested. M5I-1 does not cross-react with the human MDR1, MRP1, MRP2, or MRP3 gene products.
Immunogen
A bacterial fusion protein containing amino acids 82-168 of human MRP5Lymph node cells from an immunized rat (Wistar) fused with SP2/O mouse myleoma cells.
ABCC5
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
Immunocytochemistry: Use a 1:20 to 1:50 dilution on acetone-fixed cytospin preparations. Immunohistochemistry: Use a 1:20 dilution on acetone-fixed frozen sections, followed by incubation with rabbit anti-rat IgG and monoclonal rat APAAP complex. Alternatively, after incubation with primary antibody and washing, slides can be incubated with biotinylated rabbit anti-rat IgG and streptavidin conjugated to horseradish peroxidase. Western blotting: Use a 1:20 to 1:50 dilution (with anti-rat-HRP). The optimal dilution for a specific application should be determined by the researcher.
Restrictions
For Research Use only
Format
Liquid
Buffer
Serum free culture supernatant concentrated to 250 µg/ml, with 0.7% BSA and 0.1% sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Target
ABCC5
(ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 5 (ABCC5))