This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This mouse IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S701 of mouse IKKB.
IKBKB
Reactivity: Human
ELISA, WB, IHC, IF
Host: Rabbit
Monoclonal
3E1
unconjugated
Recombinant Antibody
Application Notes
DB: 1:500
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Busuttil, Droin, McCormick, Bernassola, Candi, Melino, Green: "NF-kappaB inhibits T-cell activation-induced, p73-dependent cell death by induction of MDM2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 42, pp. 18061-6, (2010) (PubMed).
Kenneth, Mudie, Rocha: "IKK and NF-kappaB-mediated regulation of Claspin impacts on ATR checkpoint function." in: The EMBO journal, Vol. 29, Issue 17, pp. 2966-78, (2010) (PubMed).
Tsuchiya, Asano, Nakayama, Kato, Karin, Kamata: "Nuclear IKKbeta is an adaptor protein for IkappaBalpha ubiquitination and degradation in UV-induced NF-kappaB activation." in: Molecular cell, Vol. 39, Issue 4, pp. 570-82, (2010) (PubMed).
Farlik, Reutterer, Schindler, Greten, Vogl, Müller, Decker: "Nonconventional initiation complex assembly by STAT and NF-kappaB transcription factors regulates nitric oxide synthase expression." in: Immunity, Vol. 33, Issue 1, pp. 25-34, (2010) (PubMed).
Target
IKBKB
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).