DFFB
Reactivity: Human
WB, ICC
Host: Rabbit
Polyclonal
unconjugated
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid freezing and thawing repeatly.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C for short term use.Store at -20 °C for long term preservation.
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.These death signals finally cause the degradation of chromosomal DNA by activated DNase.A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease).The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively.DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD.Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei.Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death. Synonyms: CAD (I18)