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Roundabout, Axon Guidance Receptor, Homolog 3 (Drosophila) (ROBO3) (Internal Region) antibody

Details for Product No. ABIN190793, Supplier: Log in to see
Antigen
  • Rbig1
  • Rig-1
  • Rig1
  • Robo3a
  • Robo3b
  • HGPPS
  • HGPS
  • RBIG1
  • RIG1
Epitope
Internal Region
22
9
8
4
4
2
1
Reactivity
Human
66
36
21
6
4
3
2
2
2
1
1
1
Host
Goat
51
24
6
Clonality
Polyclonal
Conjugate
Un-conjugated
6
5
5
5
4
4
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
45
39
11
10
10
4
2
Supplier
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Immunogen Peptide with sequence C-RRKQRKELSHYT, from the internal region of the protein sequence according to NP_071765.2.
Blocking Peptide Blocking peptide for this product available: ABIN369819
Cross-Reactivity Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ROBO3 / RIG1 (ROBO3 Antibody Abstract)
Background Synonyms: ROBO3, RIG1, roundabout, axon guidance receptor, homolog 3 (Drosophila), FLJ21044, HGPPS, RBIG1, retinoblastoma inhibiting gene 1, roundabout, axon guidance receptor, homolog 3
Gene ID 64221
NCBI Accession NP_071765
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 150 kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 148 kDa according to NP_071765.2). Recommended concentration: 0.5-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 0.5mg/ml
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Roundabout, Axon Guidance Receptor, Homolog 3 (Drosophila) (ROBO3) (Internal Region) antibody (ABIN190793) ABIN190793 (0.5µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RI...
Product cited in: Bauer, Dowejko, Bosserhoff et al.: "Slit-2 facilitates interaction of P-cadherin with Robo-3 and inhibits cell migration in an oral squamous cell carcinoma cell line." in: Carcinogenesis, Vol. 32, Issue 6, pp. 935-43, 2011 (PubMed). (1:50).

Background publications Kato, Takeuchi, Sato et al.: "Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses." in: Nature, Vol. 441, Issue 7089, pp. 101-5, 2006 (PubMed).