PAX6 antibody (Internal Region)
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- Target See all PAX6 Antibodies
- PAX6 (Paired Box 6 (PAX6))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PAX6 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- Purpose
- PAX6 (internal)
- Sequence
- REEKLRNQRR QASN
- Specificity
- This antibody is expected to recognize all reported isoforms: NP_000271.1, NP_001595.2, NP_001297088.1, NP_001355839.1, NP_001355840.1, NP_001355841.1, NP_001355844.1, NP_001355847.1, NP_001355849.1, NP_001355850.1, NP_001355851.1, NP_001355857.1, NP_0013
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-REEKLRNQRRQASN, from the internal region of the protein sequence according to NP_000271.1, NP_001595.2.
- Isotype
- IgG
- Top Product
- Discover our top product PAX6 Primary Antibody
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- Application Notes
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Western Blot: Approx 26 kDa band observed in lysates of cell lines U251 and RAW264.7 and in nuclear lysates of cell lines K562 and HeLa, all successfully blocked by incubation with the immunising peptide (calculated MW of 24.8 kDa according to Human NP_001
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- PAX6 (Paired Box 6 (PAX6))
- Alternative Name
- PAX6 (PAX6 Products)
- Synonyms
- pax6 antibody, an2 antibody, mgda antibody, wagr antibody, pax-6 antibody, pax6a antibody, xpax6 antibody, XLPAX6 antibody, MGC52531 antibody, PAX-6 antibody, AN antibody, AN2 antibody, D11S812E antibody, MGDA antibody, WAGR antibody, Pax-6 antibody, pax6b antibody, 1500038E17Rik antibody, AEY11 antibody, Dey antibody, Gsfaey11 antibody, Sey antibody, PAXZF-A antibody, Pax6.1 antibody, cb280 antibody, fc20e07 antibody, pax-a antibody, pax[zf-a] antibody, paxzfa antibody, wu:fc20e07 antibody, zfpax-6a antibody, zfpax-6b antibody, paired box 6 antibody, paired box 6 L homeolog antibody, paired box 6 S homeolog antibody, paired box 6a antibody, PAX6 antibody, pax6 antibody, Pax6 antibody, pax6.L antibody, pax6.S antibody, pax6a antibody
- Background
- PAX6, paired box gene 6 (aniridia, keratitis), AN, AN2, D11S812E, MGC17209, MGDA, WAGR, paired box gene 6, paired box homeotic gene 6 (aniridia, keratitis), paired box homeotic gene-6
- Gene ID
- 5080, 18508, 25509
- NCBI Accession
- NP_000271, NP_001595
- Pathways
- Peptide Hormone Metabolism, Carbohydrate Homeostasis
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