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TBC1D4 antibody (TBC1 Domain Family, Member 4) (Internal Region)

Details for Product anti-TBC1D4 Antibody No. ABIN190878, Supplier: Log in to see
Antigen
  • AS160
  • 5930406J04Rik
  • A930035N22
  • AV295684
  • As160
  • RGD1561609
Epitope
Internal Region
18
14
7
7
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
86
26
22
4
1
1
1
Host
Goat
63
18
6
Clonality
Polyclonal
Conjugate
This TBC1D4 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
58
40
26
17
16
11
9
3
3
2
2
1
Supplier
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Immunogen Peptide with sequence C-DDPEKIEERKKSK, from the internal region of the protein sequence according to NP_055647.2.
Blocking Peptide Blocking peptide for this product available: ABIN370287
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name AS160 / TBC1D4 (TBC1D4 Antibody Abstract)
Background Synonyms: TBC1D4, AS160, TBC1 domain family, member 4, RP11-159J2.1, DKFZp779C0666, Acrg embryonic lethality (mouse) minimal region ortholog, OTTHUMP00000040818, TBC (Tre-2, BUB2, CDC16) domain-containing protein, KIAA0603
Gene ID 9882
NCBI Accession NP_055647
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 150 kDa band observed in lysates of cell lines Daudi and MOLT4 (calculated MW of 147 kDa according to NP_055647.2). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Karlsson, Zierath, Kane et al.: "Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects." in: Diabetes, Vol. 54, Issue 6, pp. 1692-7, 2005 (PubMed).