RBX1 antibody (C-Term)

Catalog No. ABIN1944784

Product Details anti-RBX1 Antibody

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Binding Specificity: AA 74-108, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: E

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This RBX1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 74-108 amino acids from the C-terminal region of human RBX1.

Clone: RB48352

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-RBX1 antibody (ABIN1944784)

Ohta, Michel, Schottelius, Xiong: "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity." in: Molecular cell, Vol. 3, Issue 4, pp. 535-41, (1999) (PubMed).

Kamura, Koepp, Conrad, Skowyra, Moreland, Iliopoulos, Lane, Kaelin, Elledge, Conaway, Harper, Conaway: "Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase." in: Science (New York, N.Y.), Vol. 284, Issue 5414, pp. 657-61, (1999) (PubMed).

Target Details for RBX1

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Alternative Name: RBX1 (RBX1 Products)

Synonyms: BA554C12.1 antibody, RNF75 antibody, ROC1 antibody, 1500002P15Rik antibody, AA517855 antibody, im:7137515 antibody, zgc:136444 antibody, RBX1 antibody, ATRBX1 antibody, F7C8.160 antibody, F7C8_160 antibody, HRT1 antibody, REGULATOR OF CULLINS-1 antibody, RING-BOX 1 antibody, RING-box 1 antibody, hop21 antibody, BEST:CK01110 antibody, CG16982 antibody, CK01110 antibody, Dmel\CG16982 antibody, EG:115C2.11 antibody, Rbx1 antibody, Rbx1/Roc antibody, Roc antibody, Roc1alpha antibody, anon-EST:Posey65 antibody, dRbx1 antibody, dRoc1 antibody, dRoc1a antibody, ring-box 1 antibody, ring-box 1, E3 ubiquitin protein ligase antibody, RING-box 1 antibody, RING-box protein 1 antibody, RING-box protein 1a antibody, Regulator of cullins 1a antibody, RBX1 antibody, Rbx1 antibody, rbx1 antibody, MGG_08844 antibody, LOC9308017 antibody, rbx-1 antibody, Roc1a antibody

Background: E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

Molecular Weight: 12274

Gene ID: 9978

UniProt: P62877

Pathways: Cell Division Cycle, M Phase, SARS-CoV-2 Protein Interactome