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C-JUN antibody (Jun Proto-Oncogene) (pTyr170)

Details for Product anti-JUN Antibody No. ABIN196650, Supplier: Login to see
Antigen
  • BSK
  • BSK/DJNK
  • Bsk
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • JNK
  • JNK/SAPK
  • Jnk
  • Junk
  • SAPKa
  • c-Jun
  • dJNK
  • jnk
  • Jun
  • AP-1
  • JUN
  • B230310J22Rik
  • p39
  • Junc
  • c-jun
  • AP1
  • fj36h07
  • wu:fj36h07
  • zgc:65863
  • CG2275
  • D-Jun
  • D-jun
  • D-jun/Jra
  • DJUN
  • DJun
  • Djun
  • Dmel\\CG2275
  • JRA
  • V
  • cJun
  • d-JRA
  • d-Jun
  • d-jun
  • dAP-1
  • dJRA
  • dJUN
  • dJra
  • dJun
  • djun
  • dm-Jun
  • jra
  • jun
  • l(2)46Ef
  • l(2)IA109
  • l(2R)IA109
Alternatives
anti-Mouse (Murine) C-JUN antibody for Western Blotting
Epitope
pTyr170
71
59
44
40
40
39
38
33
22
20
20
19
14
13
13
13
13
11
8
8
7
7
6
6
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
725
378
317
44
36
30
21
17
17
12
11
8
5
5
3
2
2
2
2
1
Host
Rabbit
653
97
3
2
Clonality
Polyclonal
Conjugate
This C-JUN antibody is un-conjugated
16
16
16
12
9
9
9
7
7
7
7
7
7
7
7
Application
Western Blotting (WB)
567
321
171
108
97
84
68
65
26
20
11
10
8
6
6
3
2
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of tyrosine 170 (P-V-YP-A-N).
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. c-Jun (phospho-Tyr170) antibody detects endogenous levels of c-Jun only when phosphorylated at tyrosine 170.
Purification Immunoaffinity chromatography.
Alternative Name AP-1 / C-Jun (JUN Antibody Abstract)
Background The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.Synonyms: Activator protein 1, Proto-oncogene c-jun, Transcription factor AP1, V-jun avian sarcoma virus 17 oncogene homolog, p39
Gene ID 3725
NCBI Accession NP_002219
UniProt P05412
Research Area Transcription Factors
Pathways MAPK Signaling, RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes Suitable for use in Western blot (1: 500approx. 1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-C-JUN antibody (Jun Proto-Oncogene) (pTyr170) (ABIN196650) anti-Jun Proto-Oncogene (JUN) (pTyr170) antibody
Background publications Barilá, Mangano, Gonfloni et al.: "A nuclear tyrosine phosphorylation circuit: c-Jun as an activator and substrate of c-Abl and JNK." in: The EMBO journal, Vol. 19, Issue 2, pp. 273-81, 2000 (PubMed).

Kyriakis, Banerjee, Nikolakaki et al.: "The stress-activated protein kinase subfamily of c-Jun kinases." in: Nature, Vol. 369, Issue 6476, pp. 156-60, 1994 (PubMed).

Dérijard, Hibi, Wu et al.: "JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain." in: Cell, Vol. 76, Issue 6, pp. 1025-37, 1994 (PubMed).

Binétruy, Smeal, Karin: "Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain." in: Nature, Vol. 351, Issue 6322, pp. 122-7, 1991 (PubMed).

Smeal, Binetruy, Mercola et al.: "Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73." in: Nature, Vol. 354, Issue 6353, pp. 494-6, 1992 (PubMed).