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STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pTyr694)

Details for Product anti-STAT5A Antibody No. ABIN196697, Supplier: Login to see
Antigen
Alternatives
anti-Human STAT5A antibody for Immunohistochemistry (Frozen Sections)
Epitope
pTyr694
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Reactivity
Human, Mouse (Murine)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This STAT5A antibody is un-conjugated
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Supplier
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Immunogen Synthesized phosphopeptide derived from Human STAT5A around the phosphorylation site of Tyrosine 694 (D-G-Yp-V-K).
Specificity This antibody detects endogenous levels of STAT5A only when phosphorylated at Tyrosine 694.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name STAT5 / STAT5A (STAT5A Antibody Abstract)
Background The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT5 is activated in response to prolactine, growth hormone, GM-CSF, and IL-2.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A
Molecular Weight 90 kDa
Gene ID 6776
NCBI Accession NP_003143
UniProt P42229
Pathways JAK-STAT Signaling, RTK Signaling
Application Notes Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 M NaCl, 0.02 % Sodium Azide, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pTyr694) (ABIN196697) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibody
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pTyr694) (ABIN196697) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibody (Image 2)
Background publications Dentelli, Del Sorbo, Rosso et al.: "Human IL-3 stimulates endothelial cell motility and promotes in vivo new vessel formation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 4, pp. 2151-9, 1999 (PubMed).

Meinke, Barahmand-Pour, Wöhrl et al.: "Activation of different Stat5 isoforms contributes to cell-type-restricted signaling in response to interferons." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6937-44, 1997 (PubMed).

Gouilleux, Wakao, Mundt et al.: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994 (PubMed).