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ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr319)

Details for Product anti-ZAP70 Antibody No. ABIN196884, Supplier: Log in to see
Antigen
  • 70kDa
  • AI327364
  • MGC80670
  • MGC88962
  • mrtle
  • mur
  • SRK
  • Srk
  • STCD
  • STD
  • TZK
  • ZAP-70
  • zap70
  • ZAP70
Alternatives
anti-Human ZAP70 antibody for Western Blotting
Epitope
pTyr319
50
46
46
25
22
21
21
20
15
15
15
14
12
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
456
170
118
4
4
3
2
2
2
Host
Rabbit
295
141
15
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
29
21
16
14
10
7
5
5
4
4
4
4
4
4
4
1
Application
Immunofluorescence (IF), Western Blotting (WB)
278
162
151
82
62
44
39
37
10
7
5
4
4
3
2
Supplier
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Immunogen Synthetic phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyrosine 319 (S-P-Yp-S-D).
Specificity This antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyrosine 319.
Purification Immunoaffinity Chromatography
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70
Gene ID 7535
NCBI Accession NP_001070
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr319) (ABIN196884) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody
 image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr319) (ABIN196884) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody (Image 2)
Background publications Salomon, Ficarro, Brill, Brinker, Phung, Ericson, Sauer, Brock, Horn, Schultz, Peters: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003

Ku, Yablonski, Manser, Lim, Weiss: "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT." in: The EMBO journal, Vol. 20, Issue 3, pp. 457-65, 2001

Tang, Sawasdikosol, Chang, Burakoff: "SLAP, a dimeric adapter protein, plays a functional role in T cell receptor signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9775-80, 1999

Williams, Irvin, Sutor, Chini, Yacyshyn, Bubeck Wardenburg, Dalton, Chan, Abraham: "Phosphorylation of Tyr319 in ZAP-70 is required for T-cell antigen receptor-dependent phospholipase C-gamma1 and Ras activation." in: The EMBO journal, Vol. 18, Issue 7, pp. 1832-44, 1999

Zhao, Weiss: "Enhancement of lymphocyte responsiveness by a gain-of-function mutation of ZAP-70." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6765-74, 1997