PRKDC antibody (Protein Kinase, DNA-Activated, Catalytic Polypeptide) (pThr2609)

Details for Product anti-PRKDC Antibody No. ABIN196898, Supplier: Log in to see
Antigen
  • PRKDC
  • AI326420
  • AU019811
  • DNA-PKcs
  • DNAPDcs
  • DNAPK
  • DNPK1
  • DOXNPH
  • HYRC1
  • XRCC7
  • dxnph
  • p460
  • scid
  • slip
  • HYRC
  • p350
  • dna-pkcs
  • dnapk
  • dnpk1
  • hyrc
  • hyrc1
  • prkdc-A
  • xrcc7
  • DNA-PKCS
  • protein kinase, DNA-activated, catalytic polypeptide
  • protein kinase, DNA activated, catalytic polypeptide
  • PRKDC
  • Prkdc
  • prkdc
Alternatives
anti-Hamster PRKDC antibody for Electrophoretic Mobility-Shift Assay
Epitope
pThr2609
57
25
17
17
17
16
15
15
8
7
6
6
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
412
157
57
13
9
7
6
3
3
2
2
2
2
1
1
1
Host
Rabbit
317
99
3
1
Clonality
Polyclonal
Conjugate
This PRKDC antibody is un-conjugated
21
19
17
9
8
8
8
8
7
7
7
7
7
7
7
5
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
204
141
110
108
90
88
60
39
15
11
8
6
5
3
2
2
2
1
Options
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human DNA PKcs around the phosphorylation site of threonine 2609 (V-E-TP-Q-A).
Specificity DNA PKcs (phospho-Thr2609) antibody detects endogenous levels of DNA PKcs only when phosphorylated at threonine 2609.
Purification Immunoaffinity chromatography using epitope-specific phosphopeptide.
Alternative Name DNA-PKcs / PRKDC / XRCC7 (PRKDC Antibody Abstract)
Background DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460
Gene ID 5591
NCBI Accession NP_001075109
UniProt P78527
Pathways DNA Damage Repair, Production of Molecular Mediator of Immune Response
Application Notes Suitable for use in Suitable for use in Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (pThr2609) antibody (ABIN196898) anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (pThr2609) antibody
Background publications Ding, Reddy, Wang, Woods, Douglas, Ramsden, Lees-Miller, Meek: "Autophosphorylation of the catalytic subunit of the DNA-dependent protein kinase is required for efficient end processing during DNA double-strand break repair." in: Molecular and cellular biology, Vol. 23, Issue 16, pp. 5836-48, 2003 (PubMed).

Chan, Chen, Prithivirajsingh, Kurimasa, Story, Qin, Chen: "Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks." in: Genes & development, Vol. 16, Issue 18, pp. 2333-8, 2002 (PubMed).

Douglas, Sapkota, Morrice, Yu, Goodarzi, Merkle, Meek, Alessi, Lees-Miller: "Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase." in: The Biochemical journal, Vol. 368, Issue Pt 1, pp. 243-51, 2002 (PubMed).

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