Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (pThr2609) antibody

Details for Product No. ABIN196899
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Antigen
Synonyms PRKDC, AI326420, AU019811, DNA-PKcs, DNAPDcs, DNAPK, DNPK1, DOXNPH, HYRC1, XRCC7, dxnph, p460, scid, slip, HYRC, p350, dna-pkcs, dnapk, dnpk1, hyrc, hyrc1, prkdc-A, xrcc7, DNA-PKCS
Epitope
pThr2609
(36), (15), (14), (14), (14), (11), (8), (7), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(273), (121), (56), (40), (29), (13), (12), (4), (3), (1), (1), (1)
Host
Rabbit
(219), (58), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(15), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4)
Application
Western Blotting (WB)
(125), (82), (80), (74), (60), (46), (45), (17), (14), (9), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 50 μg
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Catalog No. ABIN196899
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human DNA PKcs around the phosphorylation site of threonine 2609 (V-E-TP-Q-A).
Specificity DNA PKcs (phospho-Thr2609) antibody detects endogenous levels of DNA PKcs only when phosphorylated at threonine 2609.
Purification Immunoaffinity chromatography using epitope-specific phosphopeptide.
Alternative Name DNA-PKcs / PRKDC / XRCC7
Background DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.
Alternate names: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460
Gene ID 5591
NCBI Accession NP_001075109
UniProt P78527
Application Notes Suitable for use in Suitable for use in Western blot (1: 500-1: 1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Supplier Images
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (pThr2609) antibody anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (pThr2609) antibody
Background publications Douglas, Sapkota, Morrice et al.: "Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase." in: The Biochemical journal, Vol. 368, Issue Pt 1, pp. 243-51, 2002 (PubMed).

Chan, Chen, Prithivirajsingh et al.: "Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks." in: Genes & development, Vol. 16, Issue 18, pp. 2333-8, 2002 (PubMed).

Ding, Reddy, Wang et al.: "Autophosphorylation of the catalytic subunit of the DNA-dependent protein kinase is required for efficient end processing during DNA double-strand break repair." in: Molecular and cellular biology, Vol. 23, Issue 16, pp. 5836-48, 2003 (PubMed).

Validation Images
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