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KIT antibody (Mast/stem Cell Growth Factor Receptor) (pTyr721)

Details for Product anti-KIT Antibody No. ABIN196992, Supplier: Log in to see
Antigen
  • Bs
  • C-Kit
  • c-kit
  • c-KIT
  • CD117
  • Fdc
  • Gsfsco1
  • Gsfsco5
  • Gsfsow3
  • MGF
  • PBT
  • SCFR
  • SCO1
  • SCO5
  • SOW3
  • Ssm
  • Tr-kit
  • W
Alternatives
anti-Human KIT antibody for Immunofluorescence
Epitope
pTyr721
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Reactivity
Human, Mouse (Murine)
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425
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Host
Rabbit
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8
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Clonality
Polyclonal
Conjugate
This KIT antibody is un-conjugated
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Application
Western Blotting (WB)
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Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human c- kit around the phosphorylation site of tyrosine 721 (N-E-YP-M-D).
Specificity C-Kit antibody detects endogenous levels of c-kit only when phosphorylated at tyrosine 721.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name CD117 / C-Kit (KIT Antibody Abstract)
Background C-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR
Gene ID 3815
NCBI Accession NP_000213
UniProt P10721
Research Area CD Antigens, Surface Receptors of Immune Cells, Growth Factors, Receptors, Proliferation Markers
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Blot: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-KIT antibody (Mast/stem Cell Growth Factor Receptor) (pTyr721) (ABIN196992) anti-Mast/stem Cell Growth Factor Receptor (KIT) (pTyr721) antibody
Background publications Blume-Jensen, Jiang, Hyman, Lee, OGorman, Hunter: "Kit/stem cell factor receptor-induced activation of phosphatidylinositol 3'-kinase is essential for male fertility." in: Nature genetics, Vol. 24, Issue 2, pp. 157-62, 2000 (PubMed).

Hirota, Isozaki, Moriyama, Hashimoto, Nishida, Ishiguro, Kawano, Hanada, Kurata, Takeda, Muhammad Tunio, Matsuzawa, Kanakura, Shinomura, Kitamura: "Gain-of-function mutations of c-kit in human gastrointestinal stromal tumors." in: Science (New York, N.Y.), Vol. 279, Issue 5350, pp. 577-80, 1998 (PubMed).

Lowe: "Colipase stabilizes the lid domain of pancreatic triglyceride lipase." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 9-12, 1997 (PubMed).

Sattler, Salgia, Shrikhande, Verma, Pisick, Prasad, Griffin: "Steel factor induces tyrosine phosphorylation of CRKL and binding of CRKL to a complex containing c-kit, phosphatidylinositol 3-kinase, and p120(CBL)." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10248-53, 1997 (PubMed).

Nocka, Tan, Chiu, Chu, Ray, Traktman, Besmer: "Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W." in: The EMBO journal, Vol. 9, Issue 6, pp. 1805-13, 1990 (PubMed).