Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
STAT5A antibody detects endogenous levels of total STAT5A protein.
Purification
Affinity-chromatography using epitope-specific immunogen
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human STAT5A around the phosphorylation site of serine 780 (R-L-SP-P-P).
STAT5A
Reactivity: Human, Mouse, Rat, Cow
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Target
STAT5A
(Signal Transducer and Activator of Transcription 5A (STAT5A))