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ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (Tyr319)

Details for Product anti-ZAP70 Antibody No. ABIN197384, Supplier: Log in to see
Antigen
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
Alternatives
anti-Human ZAP70 antibody for Western Blotting
Epitope
Tyr319
50
47
46
25
22
21
21
20
15
15
15
14
12
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
456
170
119
4
4
3
2
2
2
Host
Rabbit
295
141
15
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
29
21
16
14
10
7
5
5
4
4
4
4
4
4
4
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
278
162
151
82
61
44
39
38
10
7
5
4
4
3
2
Supplier
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Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 319 (S-P-Yp-S-D).
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Zap-70 antibody AP02691PU detects endogenous levels of total Zap-70 protein.
Purification Immunoaffinity chromatography.
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70
Gene ID 7535
NCBI Accession NP_001070
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Application Notes Suitable for use in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (Tyr319) (ABIN197384) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Tyr319) antibody
 image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (Tyr319) (ABIN197384) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Tyr319) antibody (Image 2)
Background publications Adjali, Marodon, Steinberg et al.: "In vivo correction of ZAP-70 immunodeficiency by intrathymic gene transfer." in: The Journal of clinical investigation, Vol. 115, Issue 8, pp. 2287-95, 2005 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Ku, Yablonski, Manser et al.: "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT." in: The EMBO journal, Vol. 20, Issue 3, pp. 457-65, 2001 (PubMed).

Tang, Sawasdikosol, Chang et al.: "SLAP, a dimeric adapter protein, plays a functional role in T cell receptor signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9775-80, 1999 (PubMed).

Williams, Irvin, Sutor et al.: "Phosphorylation of Tyr319 in ZAP-70 is required for T-cell antigen receptor-dependent phospholipase C-gamma1 and Ras activation." in: The EMBO journal, Vol. 18, Issue 7, pp. 1832-44, 1999 (PubMed).

Zhao, Weiss: "Enhancement of lymphocyte responsiveness by a gain-of-function mutation of ZAP-70." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6765-74, 1997 (PubMed).