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Cortactin antibody (CTTN) (Tyr421)

Details for Product anti-CTTN Antibody No. ABIN197531, Supplier: Login to see
Antigen
Alternatives
anti-Mouse (Murine) Cortactin antibody for Western Blotting
Epitope
Tyr421
30
28
17
15
14
12
9
7
7
7
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
193
108
61
8
7
5
3
2
2
1
1
1
1
1
1
Host
Rabbit
179
25
16
Clonality
Polyclonal
Conjugate
This Cortactin antibody is un-conjugated
4
4
4
4
4
4
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
182
90
86
26
21
12
10
3
3
3
3
2
1
Supplier
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Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from mouse Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Cortactin (CTTN Antibody Abstract)
Background Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1
Gene ID 10090
UniProt Q60598
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments
Pathways MAPK Signaling
Application Notes Immunohistochemistry: 1: 50-1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-Cortactin antibody (CTTN) (Tyr421) (ABIN197531) anti-Cortactin (CTTN) (Tyr421) antibody
Background publications Luo, Pan, Mines et al.: "CXCL12 induces tyrosine phosphorylation of cortactin, which plays a role in CXC chemokine receptor 4-mediated extracellular signal-regulated kinase activation and chemotaxis." in: The Journal of biological chemistry, Vol. 281, Issue 40, pp. 30081-93, 2006 (PubMed).

Head, Jiang, Li et al.: "Cortactin tyrosine phosphorylation requires Rac1 activity and association with the cortical actin cytoskeleton." in: Molecular biology of the cell, Vol. 14, Issue 8, pp. 3216-29, 2003 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Huang, Liu, Haudenschild et al.: "The role of tyrosine phosphorylation of cortactin in the locomotion of endothelial cells." in: The Journal of biological chemistry, Vol. 273, Issue 40, pp. 25770-6, 1998 (PubMed).