E-cadherin antibody

Catalog No. ABIN198433

This Mouse Monoclonal antibody specifically detects E-cadherin in WB, ELISA and Func. It exhibits reactivity toward Human.

Quick Overview for E-cadherin antibody (ABIN198433)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Product Details anti-E-cadherin Antibody

Specificity: Reacts strongly with human epithelial cadherin (E-cadherin or uvomorulin). Human, (positive control: A431 cell lysate).

Purification: Ion exchange chromatography

Immunogen: Human placenta.

Type of Immunogen: Tissue

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (1:10000), Func, WB

Usage: Suitable for use in ELISA, Western Blot. ELISA: 1:10000 (using immobilized A431 cells). Western Blot: 1 - 2 μg/mL (reducing or non-reducing condition). Inhibition of E-cadherin-dependent cell-cell contact: 5-10 μg/mL. Western Blot: 1 - 2 μg/mL. Positive control: A431 cell lysate).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 50 μL of Sterile distilled water.

Concentration: Lot specific

Buffer: Lyophilized from Tris-buffered saline, 1.0 % BSA.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1 / E Cadherin

Background: Name/Gene ID: CDH1
Family: Cadherin

Synonyms: CDH1, CD324, CDHE, Cell-CAM 120/80, E Cadherin, E-Cadherin, ECAD, Epithelial cadherin, Arc-1, UVO, Cadherin-1, CAM 120/80, CD324 antigen, LCAM, Uvomorulin

Gene ID: 999

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation