ITGB1 antibody

Catalog No. ABIN199332

Product Details anti-ITGB1 Antibody

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: Reacts with b1 subunit of VLA (b1) integrins. Species cross-reactivity: mouse.

Purification: Protein A purified

Immunogen: Integrin, beta1.

Clone: MB1-2

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: Flo (10 μg/mL), Func, IHC, IHC-Fr (20 μg/mL), IP, WB (10 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry and Western Blot. Not suitable for use in Inhibition. Immunoprecipitation: 2 μg Lysate of 1E6 cell. Flow Cytometry: 10 μg/mL. Immunohistochemistry (Frozen): 20 μg/mL. As a result of the wide tissue distribution of VLA (b1), binding to diverse cell types is commonly observed. Western Blot: 10 μg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples. Inhibition: Does not block VLA integrin mediated cell adhesion to matrix proteins. Positive control: Human OVCAR5 cell line whole cell lysate.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.25 M sodium chloride, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: ITGB1 / Integrin Beta 1 / CD29 (ITGB1 Products)

Synonyms: CD29 antibody, FNRB antibody, GPIIA antibody, MDF2 antibody, MSK12 antibody, VLA-BETA antibody, VLAB antibody, 4633401G24Rik antibody, AA409975 antibody, AA960159 antibody, ENSMUSG00000051907 antibody, Fnrb antibody, Gm9863 antibody, gpIIa antibody, cd29 antibody, fnrb antibody, gpiia antibody, itgb1 antibody, mdf2 antibody, msk12 antibody, vla-beta antibody, vlab antibody, itgb1-a antibody, itgb1-b antibody, integrin subunit beta 1 antibody, integrin beta 1 (fibronectin receptor beta) antibody, integrin subunit beta 1 S homeolog antibody, integrin beta 1 antibody, integrin subunit beta 1 L homeolog antibody, ITGB1 antibody, Itgb1 antibody, itgb1.S antibody, LOC100009173 antibody, itgb1.L antibody

Background: Name/Gene ID: ITGB1
Family: Integrin

Synonyms: ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta

Gene ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome