ZAP70 antibody
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- Target See all ZAP70 Antibodies
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ZAP70 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)
- Specificity
- Recognizes human p70 ZAP. Species cross-reactivity: Does not recognize mouse.
- Purification
- Protein G purified
- Immunogen
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Fusion Protein containing the 2 tandem SH2 domains of human ZAP-70.
Type of Immunogen: Fusion protein - Isotype
- IgG2a
- Top Product
- Discover our top product ZAP70 Primary Antibody
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- Application Notes
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Approved: Flo, IP, WB
Usage: Suitable for use in Western Blot, Flow Cytometry and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitated p70 ZAP from 500 μg of RIPA lysates from Jurkat or Hut-78 cell lines. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
- Alternative Name
- ZAP70 (ZAP70 Products)
- Synonyms
- SRK antibody, STCD antibody, STD antibody, TZK antibody, ZAP-70 antibody, 70kDa antibody, AI327364 antibody, Srk antibody, mrtle antibody, mur antibody, zap70 antibody, MGC80670 antibody, MGC88962 antibody, ZAP70 antibody, zgc:110383 antibody, zeta chain of T-cell receptor associated protein kinase 70 antibody, putative tyrosine-protein kinase zap-70 antibody, zeta-chain (TCR) associated protein kinase antibody, zeta chain of T cell receptor associated protein kinase 70 antibody, zeta-chain (TCR) associated protein kinase 70kDa antibody, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog antibody, zeta chain of T cell receptor associated protein kinase 70kDa antibody, tyrosine-protein kinase ZAP-70 antibody, ZAP70 antibody, Smp_149470 antibody, Zap70 antibody, zap70.L antibody, zap70 antibody, LOC707555 antibody, LOC100342021 antibody, LOC100390922 antibody, LOC100542877 antibody
- Background
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Name/Gene ID: ZAP70
Subfamily: Syk/ZAP70
Family: Protein Kinase
Synonyms: ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70 - Gene ID
- 7535
- UniProt
- P43403
- Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
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