HLA Class I alpha G (HLAG) antibody

Antigen: HLA Class I alpha G (HLAG)

Clonality: Monoclonal (01G)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN238362

Additional Information

Characteristics: Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen

Alternative name: HLA class I alpha G / HLA-G

Swiss-Prot: P17693

Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected withand expressing genes encoding HLA-G and human beta2-microglobulin.

Cross-Reactivity: Human

Isotype: IgG1  (Matching secondary antibodies)

Clone: 01G

Description: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of aheavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternatesplicing provides both soluble and membrane bound forms of HLA G, with two solubleisoforms and four membrane bound isoforms. HLA G may be part of the fetal protectionsystem and is expressed on fetal derived placental cells.

Specificity: The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but notsoluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical)and it is expressed on the surface of trophoblast cells. Species: Human. Others not tested.

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Immunohistochemistry (frozen sections). Immunocytochemistry. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only