HLA Class I alpha G (HLAG) antibody

Antigen: HLA Class I alpha G (HLAG)

Clonality: Monoclonal (5A6G7)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), ELISA (Capture), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN238372

Additional Information

Characteristics: Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen

Alternative name: HLA class I alpha G / HLA-G

Swiss-Prot: P17693

Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupledto ovalbumin.

Cross-Reactivity: Human

Isotype: IgG1  (Matching secondary antibodies)

Clone: 5A6G7

Description: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of aheavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternatesplicing provides both soluble and membrane bound forms of HLA G, with two solubleisoforms and four membrane bound isoforms. HLA G may be part of the fetal protectionsystem and is expressed on fetal derived placental cells.

Specificity: The antibody 5A6G7 allows to discriminate between soluble HLA-G protein yielded byshedding from membrane-bound HLA-G forms that do not contain the intron 4-encodedepitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced

Application Details

Application Notes: Western Blotting (Reducing Conditions): 12% AA SDS-PAGE. Positive Control: JEG-3 cell lysate. Flow Cytometry. Immunohistochemistry on Paraffin and Frozen Sections: 10-20 μg/ml. Positive tissue: placenta tissue. Immunocytochemistry. ELISA: The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISAfor analysis of soluble HLA-G in combination with antibody ABIN238525. Positive Control: HeLa/HLA-G5 transfectants cell lysate. HeLa/HLA-G5 cell supernatant. Negative Control: HeLa cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4 with 15 mM Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only