HLA Class I alpha G (HLAG) antibody

Antigen: HLA Class I alpha G (HLAG)

Clonality: Monoclonal (G233)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP)

Catalog no.: ABIN238406

Additional Information

Characteristics: Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen

Alternative name: HLA class I alpha G / HLA-G

Gene ID: 3135

Swiss-Prot: P17693

Immunogen: Murine L cells transfected with both human beta2-microglobulin and HLA-G

Cross-Reactivity: Human

Isotype: IgG2a  (Matching secondary antibodies)

Clone: G233

Description: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of aheavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternatesplicing provides both soluble and membrane bound forms of HLA G, with two solubleisoforms and four membrane bound isoforms. HLA G may be part of the fetal protectionsystem and is expressed on fetal derived placental cells.

Specificity: The antibody G233 recognizes several isoforms of HLA-G expressed in all populations ofextravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast,placental bed giant cells). HLA-G belongs to the nonclassical MHC Class I molecules (MHCClass Ib). The antibody G233 has been found not to cross-react with any other MHC Class Iantigens (HLA-A, -B, -C, -E, -F). Species Reactivity: Tested: Human

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing. Shelf life: one year from despatch.

Restrictions: For Research Use only