V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) antibody

Antigen: V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS)

Synonyms: CTLO, HAMSV, HRAS1, K-RAS, N-RAS, RASH1, C-H-RAS, H-RASIDX, C-BAS/HAS, C-HA-RAS1, c-H-ras, H-Ras, K-Ras, hras1, rash1, HRAS, ras, N-Ras, c-bas/has, zgc:110250, H-RAS, LOC100227892

Clonality: Monoclonal (H-Ras-03)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN238418

Additional Information

Characteristics: Synonyms: GTPase HRas, HRAS1, p21ras, H-Ras-1, c-H-ras, Ha-Ras, H-Ras, v-Ha-ras Harvey rat sarcomaviral oncogene homolog

Alternative name: HRAS

Gene ID: 3265

Swiss-Prot: P01112

Immunogen: Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein.

Cross-Reactivity: Human

Isotype: IgG1  (Matching secondary antibodies)

Clone: H-Ras-03

Description: H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellularleaflet of the plasma membrane to regulate multiple signal transduction pathways, such asmitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasmamembrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity forlipid rafts and allows the protein to target to nonraft microdomains, the primary sites ofH-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyzedissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras alsodiffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependenton Ras palmitoylation.

Specificity: The antibody H-Ras-03 reacts with human H-Ras, an ubiquitously expressed 21 kDaintracellular protein. Although reactivity with other species has not been determined, it isprobable as the epitope is highly conserved among animals.

Application Details

Application Notes: ELISA. Western Blotting under Reducing conditions (0.5-1 μg/ml): Positive control: RAJI human Burkitt lymphoma cell lineHeLa human cervix carcinoma cell lineK567 human leukemia cell lineSample preparation: Resuspend approx.50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid freeze/thaw cycles. Shelf life: One year from despatch.

Restrictions: For Research Use only