Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
Specificity
The antibody Hs-14 reacts with a 220 kDa testis-specific intra-acrosomal protein associated with the membranes of the acrosomal vesicle.
Characteristics
Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
Purification
Precipitation methods and size-exclusion chromatography
Purity
> 95 % (by SDS-PAGE)
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.
ACRV1
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
Flow Cytometry. Western Blotting. Immunocytochemistry: 10 μg/mL, Staining technique: Membrane permeabilization(acetone) is essential. Application note: The antibody Hs-14 is designed for quantitativeimmunofluorescence analysis of pathological sperms (excellent tool for laboratories ofassisted reproduction when optimal method of fertilization is sought). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store the antibody at 2 - 8 °C. DO NOT FREEZE! Shelf life: one year from despatch.
One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2