NODAL (Internal Region) antibody

Details for Product No. ABIN238582, Supplier: Log in to see
Antigen
  • Tg.413d
  • HTX5
  • nodal
  • nodal growth differentiation factor
  • nodal homolog (mouse)
  • Nodal
  • NODAL
Epitope
Internal Region
12
10
6
4
4
3
2
1
1
1
1
1
1
1
1
Reactivity
Human
67
28
27
7
7
6
4
4
4
3
3
1
1
1
Host
Goat
31
21
15
1
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
ELISA
35
32
14
13
6
6
4
2
Options
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Immunogen Peptide with sequence ECPNPVGEEFH, from the internal region of the protein sequence according to NP_060525.2.
Blocking Peptide Blocking peptide for this product available: ABIN370106
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Serum-free
Background Synonyms: NODAL, nodal homolog (mouse), MGC138230, mouse nodal homolog precursor, nodal, mouse, homolog
Gene ID 4838, 18119
NCBI Accession NP_060525
Application Notes ELISA: antibody detection limit dilution 1:8000. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NODAL captured by a rabbit antibody. Recommended reporter concentration: 1-2 µg/mL
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Hendrix, Seftor, Seftor, Kasemeier-Kulesa, Kulesa, Postovit: "Reprogramming metastatic tumour cells with embryonic microenvironments." in: Nature reviews. Cancer, Vol. 7, Issue 4, pp. 246-55, 2007 (PubMed).