GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN2451988, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
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Host
Rat
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Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is un-conjugated
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Application
ChIP DNA-Sequencing (ChIPSeq), Immunochromatography (IC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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Options
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Immunogen Recombinant GFP protein
Clone 1A5
Isotype IgG1 kappa
Specificity Specific to GFP and GFP-fused proteins
Characteristics The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.
Purification Purified
Sterility Sterile filtered
Alternative Name GFP
Background The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light. In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization.
UniProt P42212
Research Area Tags/Labels
Application Notes 1. Western blotting: ~1 g/mL
2. Immunoprecipitation
3. Immunocytochemistry
4. Chromatin Immunoprecipitation (ChIP)
5. ELISA
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol
Preservative Azide free
Storage -20 °C/-80 °C
Storage Comment -20 C (For long term storage: -80 C)
Supplier Images
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN2451988) anti-Green Fluorescent Protein (GFP) antibody
SDS-PAGE (SDS) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN2451988) anti-Green Fluorescent Protein (GFP) antibody (Image 2)
SDS-PAGE (SDS) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN2451988) anti-Green Fluorescent Protein (GFP) antibody (Image 3)
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN2451988) anti-Green Fluorescent Protein (GFP) antibody (Image 4)
Background publications Tsien: "The green fluorescent protein." in: Annual review of biochemistry, Vol. 67, pp. 509-44, 1998 (PubMed).

Product cited in: SHIMOMURA, JOHNSON, SAIGA: "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea." in: Journal of cellular and comparative physiology, Vol. 59, pp. 223-39, 1970 (PubMed).