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GFP antibody (Green Fluorescent Protein) Primary Antibody

GFP Reactivity: Chemical ELISA, WB Host: Mouse Monoclonal 5E5G11 unconjugated
Pubmed (6)
Catalog No. ABIN1573884
$280.57
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  • Target
    GFP
    Reactivity
    Chemical
    • 190
    • 7
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Host
    Mouse
    • 77
    • 75
    • 32
    • 16
    • 12
    • 7
    • 3
    • 1
    Clonality
    Monoclonal
    • 130
    • 89
    Conjugate
    This GFP antibody is un-conjugated
    • 121
    • 11
    • 10
    • 10
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Application
    ELISA, Western Blotting (WB)
    • 156
    • 92
    • 49
    • 47
    • 37
    • 36
    • 32
    • 22
    • 14
    • 12
    • 10
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Specificity
    The antibody reacts with various forms of GFP including eGFP, OFP, and GFPuv.
    Purification
    Protein G affinity column
    Immunogen
    Purified recombinant cGFP fusion protein
    Clone
    5E5G11
    Isotype
    IgG
  • Application Notes
    The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and length of incubationsELISA: 0.1-1.0 µg/mL
    Western blot: 1.0-2.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 4 µg of this antibody is mixed with 4 mL of WB solution.
    Other applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Li, Shen, Sun, Wang, Li, Huang, Chen, Li, Zhao, Luo, Li, Qiu: "Effects of the nuclear localization of the N(pro) protein of classical swine fever virus on its virulence in pigs." in: Veterinary microbiology, Vol. 174, Issue 3-4, pp. 391-8, 2014 (PubMed).

    Liu, Yu, Wu, Cao, Wu, Wu, Wang: "Characterization of the open reading frame 7a from Bombyx mori nucleopolyhedrovirus." in: Molecular biology reports, Vol. 40, Issue 2, pp. 865-73, 2013 (PubMed).

    Liu, Zhu, Wu, He, Chen: "Inhibition effect produced by dominant negative mutant fusion protein PreS2-TLM-ScFv-HBcDN on HBV replication in vitro." in: Journal of viral hepatitis, Vol. 19, Issue 4, pp. 295-300, 2012 (PubMed).

    Wilusz, JnBaptiste, Lu, Kuhn, Joshua-Tor, Sharp: "A triple helix stabilizes the 3' ends of long noncoding RNAs that lack poly(A) tails." in: Genes & development, Vol. 26, Issue 21, pp. 2392-407, 2012 (PubMed).

    Yang, Jin, Chen, Lin, Wang, Chen, Han, Bian, Zhu, Wang: "A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial death and lysis is required for chloroplast development in Arabidopsis thaliana." in: The New phytologist, Vol. 193, Issue 1, pp. 81-95, 2011 (PubMed).

    Qi, Wang, Shen, Zhang, Chen, Xu, Liu, Wu, Jiang: "OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa)." in: The New phytologist, Vol. 193, Issue 1, pp. 109-20, 2011 (PubMed).

  • Target
    GFP
    Alternative Name
    cGFP
    Synonyms
    green fluorescent protein, gfp
    Background
    The coral green fluorescent protein (cGFP) from vector pRNATin-H1.2/Neo (Antibodies-Online, SD1223) is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. Mouse Anti-cGFP-tag Monoclonal Antibody can be used to detect cGFP fusion protein in Western blot analyses.
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