INSIG1 antibody
-
- Target See all INSIG1 Antibodies
- INSIG1 (Insulin Induced Gene 1 (INSIG1))
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This INSIG1 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA
- Purification
- Antibody is purified by peptide affinity chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human INSIG1.
- Top Product
- Discover our top product INSIG1 Primary Antibody
-
-
- Application Notes
- INSIG1 antibody can be used for detection of INSIG1 by ELISA at 1:62500. INSIG1 antibody can be used for detection of INSIG1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store INSIG1 antibody at -20 °C.
-
- Target
- INSIG1 (Insulin Induced Gene 1 (INSIG1))
- Alternative Name
- INSIG1 (INSIG1 Products)
- Synonyms
- cl-6 antibody, cl6 antibody, CL-6 antibody, CL6 antibody, fb55a03 antibody, fi35b10 antibody, wu:fb55a03 antibody, wu:fi35b10 antibody, zgc:55439 antibody, 1810013C12Rik antibody, Insig-1 antibody, INSIG-1 antibody, insulin induced gene 1 antibody, insulin induced gene 1 L homeolog antibody, INSIG1 antibody, insig1 antibody, insig1.L antibody, Insig1 antibody
- Background
- Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
- Molecular Weight
- 36 kDa
- Gene ID
- 3638
- NCBI Accession
- NP_938150
- UniProt
- A4D2M9
- Pathways
- ER-Nucleus Signaling
-