NFE2
Reactivity: Human
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 555
Application Notes
NFE2 antibody can be used for detection of NFE2 by ELISA at 1:312500. NFE2 antibody can be used for detection of NFE2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFE2 antibody at -20 °C.
NFE2 is required for activity at the locus control region (LCR) upstream of the globin gene complexes.It requires p18 NF-E2 for binding to the NF-E2 motif. NFE2 may play a role in all aspects of hemoglobin production: globin synthesis, heme synthesis, and the procurement of iron.