DIO2 antibody (Internal Region)

Catalog No. ABIN2466502

Product Details anti-DIO2 Antibody

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DIO2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Specificity: This antibody is expected to recognize both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).

Purification: DIO2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: DIO2 antibody was raised against a 12 amino acid synthetic peptide near the internal region of DIO2.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 65-70 kDa band in human heart and thyroid lysates after 0.05 μg/mL antibody staining.5 kDa according to NP_NP_000784.2 and of 34.6 kDa according to NP_001007024.1. The 65-70 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: Cryo-sectioned mouse hippocampus shows staining in the granular layer of the dentate gyrus. Recommended concentration, 2-3 μg/mL. Data obtained from anonymous customer.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for DIO2

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Alternative Name: DIO2 (DIO2 Products)

Synonyms: 5DII antibody, D2 antibody, DIOII antibody, SelY antibody, TXDI2 antibody, AI324267 antibody, Deio2 antibody, cb684 antibody, DIO2 antibody, dio2-A antibody, 5dii antibody, sely antibody, txdi2 antibody, iodothyronine deiodinase 2 antibody, deiodinase, iodothyronine, type II antibody, deiodinase, iodothyronine, type 2 L homeolog antibody, deiodinase, iodothyronine, type 2 antibody, type II iodothyronine deiodinase antibody, DIO2 antibody, Dio2 antibody, dio2 antibody, dio2.L antibody, LOC100861220 antibody

Gene ID: 1734

NCBI Accession: NP_000784, NP_054644, NP_001007024

UniProt: Q92813

Pathways: Hormone Transport, Hormone Activity