Thioredoxin Domain-Containing Protein 4 (ERP44) antibody

Antigen: Thioredoxin Domain-Containing Protein 4 (ERP44)

Synonyms: PDIA10, TXNDC4, KIAA0573, Txndc4, AI849526, AL033348, 1110001E24Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN263212

Additional Information

Alternative name: TXNDC4

Gene ID: 23071, 76299 (mouse), 298066 (rat)

Immunogen: C-EQKDSDNYRVFER

Format: Affinity-purified

Description: All names and symbols: TXNDC4 , thioredoxin domain containing 4 (endoplasmic reticulum) , ERP44, KIAA0573, OTTHUMP00000063799, endoplasmic reticulum resident protein 44 kDa. Official Symbol: TXNDC4 . RefSeq number(s): NP_055866.1

Specificity: Human, Mouse, Rat, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 47kDa band observed in lysates of cell line K562 (calculated MW of 47.0kDa according to NP_055866.1). Recommended concentration: 0.2-0.6µg/ml. An additional band of unknown i

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN263212 (0.2 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Anelli, Ceppi, Bergamelli et al.: "Sequential steps and checkpoints in the early exocytic compartment during secretory IgM biogenesis." in: The EMBO journal, Vol. 26, Issue 19, pp. 4177-88, 2007 (PubMed).