Endoplasmic Reticulum Protein 44 (ERP44) (Internal Region) antibody

Details for Product No. ABIN1048579
Target
Binding Specificity
Internal Region
10
4
3
2
2
1
1
1
1
Reactivity
Bat, Chicken, Cow, Dog, Guinea Pig, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Xenopus laevis
38
6
5
2
2
2
2
2
2
2
2
2
2
1
1
Host
Rabbit
24
8
7
Clonality
Polyclonal
Conjugate
Un-conjugated
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
27
18
10
9
8
3
1
1
1
Options
Brand IHC-plus™
Immunogen Synthetic 20 amino acid peptide from internal region of human ERP44 / TXNDC4. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%), Opossum, Stickleback, Medaka, Pufferfish, Zebrafish (95%).

Type of Immunogen: Synthetic peptide
Specificity Human ERP44 / TXNDC4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%) Opossum, Stickleback, Medaka, Pufferfish, Zebrafish (95%).
Purification Immunoaffinity purified
Target
Alternative Name ERP44 (ERP44 Antibody Abstract)
Background Name/Gene ID: ERP44

Synonyms: ERP44, ER protein 44, PDIA10, TXNDC4, KIAA0573
Gene ID 23071
Pathways Cell RedoxHomeostasis, SARS-CoV-2 Protein Interactome
Application Notes Approved: IHC, IHC-P (15 - 20 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months
Images
Image no. 1 for anti-Endoplasmic Reticulum Protein 44 (ERP44) (Internal Region) antibody (ABIN1048579) Anti-ERP44 / TXNDC4 antibody ABIN1048579 IHC staining of human placenta. Immunohisto...
Image no. 2 for anti-Endoplasmic Reticulum Protein 44 (ERP44) (Internal Region) antibody (ABIN1048579) Anti-ERP44 / TXNDC4 antibody ABIN1048579 IHC staining of human stomach. Immunohistoc...