IL1R1 antibody (Extracellular Domain)

Catalog No. ABIN263862

The Mouse Monoclonal anti-IL1R1 antibody (Clone BDI204) (ABIN263862) specifically detects IL1R1 in RIA, IF and EIA. The antibody is reactive with Human samples.

Quick Overview for IL1R1 antibody (Extracellular Domain) (ABIN263862)

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL1R1 antibody is un-conjugated

Application: Radioimmunoassay (RIA), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: BDI204

Product Details anti-IL1R1 Antibody

Binding Specificity: Extracellular Domain

Specificity: This antibody reacts with cell surface and soluble human IL-1 receptor. Does not block binding of IL-1 to receptor. Does not cross react with receptors for human IL-2 or IL-4.

Characteristics: Synonyms: Interleukin-1 receptor type I, IL-1R-1, IL-1RT1, IL-1R-alpha, p80, IL1RA, IL1RT1, CD121antigen-like family member A

Purification: Protein G chromatography

Immunogen: The extracellular domain of the type I human IL-1 receptor

Isotype: IgG2a

Application Details

Application Notes: ELISA: Can be used as capture reagents or second antibody at a concentration of 5g/mL. RIA: Concentrations of 20-30 ng/mL of these antibodies will bind radiolabeled IL-1 receptorin a radioimmunoassay format. Immunohistochemistry: IL-1 receptor can be detected on cultured cells using antibodyconcentrations of 10-100g/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.2mg/mL (OD280nm)

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Alternative Name: CD121a / IL1R1

Background: IL1 binds antigen CD121a, which is a transmembrane glycoprotein and a member of the immunoglobulin superfamily expressed on T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells and neural cells. CD121a binds the mature forms of IL1 alpha, IL1 beta, and IL1 Ra proteins. High affinity binding and signalling by the type I IL1 receptor requires CD121a and the accessory protein, IL1 R AcP, which does not bind IL1 alone, but forms a dimer capable of binding IL1. Interleukin 1 (IL1), which has a role as a mediator in inflammation, consists of 2 separate but related proteins, IL1 alpha and IL1 beta. Cell surface receptors for the 2 forms of IL1 are identical. IL1R Type I contains a single membrane spanning segment, a large cytoplasmic region, and an extracellular, IL1 binding portion composed of 3 immunoglobulin like domains.Synonyms: IL-1R-alpha, IL1R, IL1RA, IL1RT1, Interleukin-1 receptor alpha, Interleukin-1 receptor type 1, Interleukin-1 receptor type I

Gene ID: 3554

UniProt: P14778

Pathways: NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints