Cullin 2 antibody

Catalog No. ABIN265376

Product Details anti-Cullin 2 Antibody

Target: Cullin 2 (CUL2)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Cullin-2 protein (region surrounding Ile726).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cullin 2

Target: Cullin 2 (CUL2)

Alternative Name: Cullin-2 (CUL2 Products)

Synonyms: cul2 antibody, Cullin-2 antibody, fj48a06 antibody, wu:fj48a06 antibody, cullin-2 antibody, LOC100221330 antibody, 1300003D18Rik antibody, 4932411N15Rik antibody, AI327301 antibody, mKIAA4106 antibody, ATCUL2 antibody, F22D16.2 antibody, F22D16_2 antibody, cullin 2 antibody, cullin 2 antibody, cullin-2 antibody, putative cullin-2 (cul-2) antibody, cullin 2 L homeolog antibody, Cullin-2 antibody, cul2 antibody, CUL2 antibody, PTRG_05231 antibody, Smp_079740 antibody, LOC100550749 antibody, Cul2 antibody, cul2.L antibody, cul-2 antibody

Background: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF(Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.Synonyms: CUL-2, CUL2

Molecular Weight: approx. 82, 84 kDa

Gene ID: 8453

NCBI Accession: NP_003582

UniProt: Q13617

Pathways: M Phase, Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome