TUBA1A antibody (Tubulin, alpha 1a) (Alexa Fluor 488)

Details for Product anti-Tuba1a Antibody No. ABIN2656796, Supplier: Log in to see
Antigen
  • tuba1a
  • AC195340.3_FG001
  • tua3
  • TUBA3D
  • TUBA3E
  • B-ALPHA-1
  • LIS3
  • TUBA3
  • Tuba-1
  • Tuba1
  • K-ALPHA-1
  • TUBA1
  • TUBA6
  • M[a]7
  • Tuba7
  • Tuba3a
  • RGD1565155
  • TUBA2
  • tubulin, alpha 1a
  • tubulin alpha-1C chain-like
  • Alpha-tubulin 3
  • tubulin alpha-3 chain-like
  • tubulin, alpha 1A
  • tubulin, alpha 1b
  • tubulin, alpha 1c
  • tubulin, alpha 3B
  • tubulin, alpha 3e
  • uncharacterized LOC100125553
  • TUBA1A
  • LOC100197513
  • TUBA3
  • LOC425049
  • Tuba1a
  • TUBA1B
  • TUBA1C
  • Tuba3b
  • TUBA3E
  • LOC100125553
Alternatives
anti-Human TUBA1A antibody for Immunofluorescence (fixed cells)
Reactivity
All Species, Human, Mouse (Murine), Rat (Rattus)
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Host
Mouse
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45
11
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This TUBA1A antibody is conjugated to Alexa Fluor 488
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Application
Flow Cytometry (FACS)
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Options
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Clone 10D8
Isotype IgM kappa
Purification The antibody was conjugated with Alexa Fluor® 488 under optimal conditions.
Purity The antibody is at > 85 % purity.
Alternative Name Tubulin-alpha (Tuba1a Antibody Abstract)
Background Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.
Research Area Cytoskeleton, Cell/Tissue Markers
Pathways Microtubule Dynamics, M Phase
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer phosphate-buffered solution, pH 7.4, containing 0.09 % sodium azide at 0.5 mg/mL.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C
Background publications Chou, Chou, Lee: "CDK-dependent activation of poly(ADP-ribose) polymerase member 10 (PARP10)." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15201-7, 2006 (PubMed).

Miller, Naus, Durand, Bloom, Milner: "Isotypes of alpha-tubulin are differentially regulated during neuronal maturation." in: The Journal of cell biology, Vol. 105, Issue 6 Pt 2, pp. 3065-73, 1988 (PubMed).

Cowan, Dobner, Fuchs, Cleveland: "Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions." in: Molecular and cellular biology, Vol. 3, Issue 10, pp. 1738-45, 1984 (PubMed).

Wilde, Chow, Wefald, Cowan: "Structure of two human alpha-tubulin genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 1, pp. 96-100, 1982 (PubMed).