CD4 antibody

Catalog No. ABIN2689232

Product Details anti-CD4 Antibody

Target: CD4

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Characteristics: The OX-35 clone has been reported to react with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e. MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, some dendritic cells, and microglia. CD4 is an antigen coreceptor on the T-cell surface that interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through it's association with the T-cell receptor complex and protein tyrosine kinase Lck. The OX-35 clone has been reported to bind to a different epitope of CD4 than that recognized by the W3/25 and OX-38 clones. Immunohistochemical staining of CD4+ T lymphocytes. Frozen sections of normal rat spleen were stained with the anti-rat CD4 clone OX-35 antibody. CD4+ T lymphocytes in the periarteriolar sheath are identified by the brown staining.

BD Pharmingen™ Purified Mouse Anti-Rat CD4 - Purified - Clone OX-35 - Isotype Mouse IgG2a, κ - Reactivity Rat - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat T-cell blasts

Clone: OX

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 62.5 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD4 antibody (ABIN2689232)

McElwee, Spiers, Oliver: "Partial restoration of hair growth in the DEBR model for Alopecia areata after in vivo depletion of CD4+ T cells." in: The British journal of dermatology, Vol. 140, Issue 3, pp. 432-7, (2000) (PubMed).

Morrison, Kennedy, Offner, Vandenbark: "Effects of anti-CD4 antibody: enhancement of lymph node PPD-memory T cell response." in: Cellular immunology, Vol. 163, Issue 1, pp. 106-12, (1995) (PubMed).

Bañuls, Alvarez, Ferrero, Zapata, Ardavin: "Cell-surface marker analysis of rat thymic dendritic cells." in: Immunology, Vol. 79, Issue 2, pp. 298-304, (1993) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, (1985) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4 (CD4 Products)

Synonyms: cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

Background: Synonyms: Cd4, CD4 antigen, p55, W3/25 antigen, T-cell surface glycoprotein CD4

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events