Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Cytotoxicity Test (CyTox), BioImaging (BI)
Brand
BD Pharmingen™
Characteristics
The MC480 monoclonal antibody reacts with Stage-Specific Embryonic Antigen-1 (SSEA-1), which is a terminal carbohydrate epitope (3-fucosyl-N-acetyllactosamine or 3-FAL) on glycoproteins and lactose series glycolipids. SSEA-1 is related to Lewis blood group antigens and is found in a variety of embryonic and adult tissues and cancers. As its name implies, the expression of SSEA-1 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. However, its expression pattern differs in the human and mouse. In the human, SSEA-1 is not found on embryonic stem (ES) cells, embryonic inner cell mass (ICM), or teratocarcinoma (embryonal carcinoma or EC) cells. As human EC and ES cells undergo differentiation, SSEA-1 expression is upregulated. In the adult, the same epitope is expressed as CD15 on granulocytes and monocytes, but not lymphocytes or dendritic cells. In the mouse, SSEA-1 is found on EC, ES, and primordial germ cells, 8-cell to blastocyst embryos, ICM, and on subpopulations of cells in the adult central nervous system, including stem cells. In contrast to the human, SSEA-1 expression is reduced as mouse EC and ES cells undergo differentiation. Western Blot analysis of SSEA-1 in mouse ES cell line. Lysate from ES-E14TG2a cells (ATCC CRL-1821) was probed with Purified Mouse anti-SSEA-1 monoclonal antibody at titrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 μg/mL (lane 3). High-molecular-weight molecules bearing the SSEA-1 epitope are identified above 200 kDa. Immunofluorescent staining of mouse ES cell line. ES-E14TG2a cells were cultured, fixed, and stained with Purified Mouse anti-SSEA-1 monoclonal antibody (pseudo-colored green) according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor® 647 goat anti-mouse Ig (Invitrogen) and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer using a 20X objective and merged using BD Attovision™ software. 560079 Rev. 2 Page 1 of 2
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
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