RPS6 antibody

Catalog No. ABIN272077

Product Details anti-RPS6 Antibody

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of RS6 protein. (region surrounding Leu234)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name: RPS6 (RPS6 Products)

Synonyms: S6 antibody, rps6 antibody, rps6a antibody, S6R antibody, (Rp)S6 antibody, CG10944 antibody, DS6 antibody, Dmel\\CG10944 antibody, M(1)7BC antibody, M(1)7C antibody, RPS6 antibody, Rp S6 antibody, Rps6 antibody, air8 antibody, air[8] antibody, anon-WO02059370.61 antibody, hen antibody, l(1)air8 antibody, l(1)air[8] antibody, l(1)hen antibody, pp30 antibody, rpS6 antibody, wu:fa92e06 antibody, wu:fb64g06 antibody, zgc:92237 antibody, ribosomal protein S6 antibody, ribosomal protein S6 S homeolog antibody, S6 ribosomal protein antibody, Ribosomal protein S6 antibody, 30S ribosomal protein S6 antibody, 40S ribosomal protein S6 antibody, RPS6 antibody, rps6.S antibody, LOC100135859 antibody, Rps6 antibody, RpS6 antibody, rps6 antibody, rps-6 antibody, LOC100533219 antibody

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33

Molecular Weight: approx. 32 kDa

Gene ID: 6194

NCBI Accession: NP_001001

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly