SMAD4 antibody

Catalog No. ABIN272224

Product Details anti-SMAD4 Antibody

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Smad4 protein. (region surrounding Leu43)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

References for anti-SMAD4 antibody (ABIN272224)

Nagappan, Park, Park, Kim, Hong, Kang, Zhang, Kim, Lee, Won, Kim: "Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cells." in: BMC biochemistry, Vol. 14, pp. 24, (2014) (PubMed).

Yang, Zhao, Rasul, Qin, Li, Li: "PIAS1-modulated Smad2/4 complex activation is involved in zinc-induced cancer cell apoptosis." in: Cell death & disease, Vol. 4, pp. e811, (2013) (PubMed).

Yang, Li, Wang, Wang, Li, Yang, Peng: "Smad4 disruption accelerates keratinocyte reepithelialization in murine cutaneous wound repair." in: Histochemistry and cell biology, Vol. 138, Issue 4, pp. 573-82, (2012) (PubMed).

Yan, Zhang, Fang, Zhang, Wu, Jiang, Sun, Hu: "MicroRNA-145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB." in: FEBS letters, Vol. 586, Issue 19, pp. 3263-70, (2012) (PubMed).

Izumi, Nakamura, Tokumo, Mano: "A minute pancreatic ductal adenocarcinoma with lipomatous pseudohypertrophy of the pancreas." in: JOP : Journal of the pancreas, Vol. 12, Issue 5, pp. 464-8, (2011) (PubMed).

Yuan, Wang, Hu, Li, Jing: "Transforming growth factor-?/Smad signaling function in the aortopathies." in: Revista brasileira de cirurgia cardiovascular : órgão oficial da Sociedade Brasileira de Cirurgia Cardiovascular, Vol. 26, Issue 3, pp. 393-403, (2011) (PubMed).

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4 (SMAD4 Products)

Synonyms: smad4 antibody, DPC4 antibody, JIP antibody, MADH4 antibody, MYHRS antibody, AW743858 antibody, D18Wsu70e antibody, Madh4 antibody, XSmad4alpha antibody, Xsmad4 antibody, dpc4 antibody, jip antibody, madh4 antibody, xsmad4a antibody, SMAD family member 4 antibody, Smad4 protein antibody, SMAD family member 4, gene 1 L homeolog antibody, SMAD4 antibody, smad4 antibody, Smad4 antibody, smad4.1.L antibody

Background: Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.Synonyms: DPC4, Deletion target in pancreatic carcinoma 4, MAD homolog 4, MADH4, Mothers against DPP homolog 4, Mothers against decapentaplegic homolog 4, SMAD 4, SMAD family member 4, SMAD-4

Molecular Weight: approx. 60 kDa

Gene ID: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy