PAG1 antibody (N-Term)

Catalog No. ABIN2745550

Product Details anti-PAG1 Antibody

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Binding Specificity: AA 412-424, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Recognizes mouse, rat and human PAG/Cbp.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Antigen affinity purified.

Immunogen: Synthetic peptide corresponding to aa 412-424 of the N-terminal rat PAG including an additional N-terminal Cys residue.

Application Details

Application Notes: Positive Control for Western Blot: Use 1.5x106 total splenocytes per lane.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for PAG1

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Alternative Name: PAG/Cbp (PAG1 Products)

Synonyms: Cbp antibody, F730007C19Rik antibody, Pag antibody, CBP antibody, PAG antibody, MGC80192 antibody, phosphoprotein associated with glycosphingolipid microdomains 1 antibody, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 antibody, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog antibody, Pag1 antibody, PAG1 antibody, pag1.L antibody, pag1 antibody

Background: PAG is a ubiquitously expressed transmembrane adapter protein. Depending on its phosphorylation status, it appears as diffuse band(s) of 80-90 kDa by SDS-PAGE, with phosphorylation leading to a shift towards a higher apparent molecular mass. Tyrosine phosphorylated PAG recruits Csk to the membrane and negatively regulates Src family kinases via Csk-mediated phosphorylation. Upon T cell receptor engagement, PAG becomes dephosphorylated, thereby displacing Csk from the membrane and enabling the activation of the Src kinases Fyn and Lck. PAG negatively regulates TCR (T cell antigen receptor)-mediated signaling in T cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. It promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. It inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins and may be involved in cell adhesion signaling.

UniProt: Q9JM80

Pathways: p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events