MBD3 antibody (N-Term)

Catalog No. ABIN2778641

Product Details anti-MBD3 Antibody

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SKMNKSRQRV RYDSSNQVKG KPDLNTALPV RQTASIFKQP VTKITNHPSN

Predicted Reactivity: Cow: 85%, Dog: 100%, Guinea Pig: 100%, Horse: 79%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MBD3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MBD3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 291 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD3 (MBD3 Products)

Synonyms: xmbd3 antibody, MGC69548 antibody, MBD3 antibody, DKFZp459N1635 antibody, AI181826 antibody, AU019209 antibody, methyl-CpG binding domain protein 3 S homeolog antibody, methyl-CpG binding domain protein 3 antibody, mbd3.S antibody, mbd3 antibody, MBD3 antibody, Mbd3 antibody

Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71 % and 94 % identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71 % and 94 % identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: -
Protein Interaction Partner: SDCBP, KRT15, ICA1, GOLGA2, CEP76, GATAD2B, RCN3, TRIM54, ZNF277, CEP250, ZBTB16, UBC, RARA, KDM1A, HDAC2, HDAC1, MMS19, HIST3H3, GATAD2A, INO80, MTA2, BAZ1B, SMARCA5, RBBP7, RBBP4, HNRNPM, IGBP1, FUS, EWSR1, DDX3X, DDB1, CHD4, CHD3, TAB2, DNMT1, MTA1, NR
Protein Size: 291

Molecular Weight: 33 kDa

Gene ID: 53615

NCBI Accession: NM_003926, NP_003917

UniProt: O95983

Pathways: Chromatin Binding