SMAD4 antibody (Middle Region)
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- Target See all SMAD4 Antibodies
- SMAD4 (SMAD Family Member 4 (SMAD4))
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Binding Specificity
- Middle Region
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SMAD4 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- NIPVASTSQP ASILGGSHSE GLLQIASGPQ PGQQQNGFTG QPATYHHNST
- Predicted Reactivity
- Cow: 100%, Dog: 92%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 100%, Sheep: 100%
- Characteristics
- This is a rabbit polyclonal antibody against SMAD4. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Protein A purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human SMAD4
- Top Product
- Discover our top product SMAD4 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 552 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- SMAD4 (SMAD Family Member 4 (SMAD4))
- Alternative Name
- SMAD4 (SMAD4 Products)
- Synonyms
- smad4 antibody, DPC4 antibody, JIP antibody, MADH4 antibody, MYHRS antibody, AW743858 antibody, D18Wsu70e antibody, Madh4 antibody, XSmad4alpha antibody, Xsmad4 antibody, dpc4 antibody, jip antibody, madh4 antibody, xsmad4a antibody, SMAD family member 4 antibody, Smad4 protein antibody, SMAD family member 4, gene 1 L homeolog antibody, SMAD4 antibody, smad4 antibody, Smad4 antibody, smad4.1.L antibody
- Background
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SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction
Alias Symbols: JIP, DPC4, MADH4, MYHRS
Protein Interaction Partner: RASSF5, LMO4, SMAD3, SMAD2, SKIL, UBC, NEDD8, SUMO1, SMURF2, SMURF1, TRIM33, CRYAB, COPS5, NLK, OTUB1, SMAD9, UBE2I, FHL3, FHL2, FHL1, CSNK1D, TGFB1I1, SMAD1, NCOR1, SKI, KLF5, IRF7, IRF3, ZNF521, ZNF423, SOX12, SNRNP70, RPL28, RFX1, RELA, RALA, PSMD11, P
Protein Size: 552 - Molecular Weight
- 60 kDa
- Gene ID
- 4089
- NCBI Accession
- NM_005359, NP_005350
- UniProt
- Q13485
- Pathways
- Cell Division Cycle, Chromatin Binding, Autophagy
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