POLR2B antibody (N-Term)
-
- Target See all POLR2B Antibodies
- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
-
Binding Specificity
- N-Term
-
Reactivity
- Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This POLR2B antibody is un-conjugated
-
Application
- Western Blotting (WB)
- Sequence
- TYSAPLYVDI TKTVIKEGEE QLQTQHQKTF IGKIPIMLRS TYCLLNGLTD
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%
- Characteristics
- This is a rabbit polyclonal antibody against POLR2B. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Protein A purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human POLR2B
- Top Product
- Discover our top product POLR2B Primary Antibody
-
-
- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
-
Antigen size: 1174 AA
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
-
- Target
- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
- Alternative Name
- POLR2B (POLR2B Products)
- Synonyms
- Pol2rb antibody, Rpb140 antibody, Rpb2 antibody, POL2RB antibody, RPB2 antibody, hRPB140 antibody, hsRPB2 antibody, RNA polymerase II subunit B antibody, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa antibody, DNA-directed RNA polymerase II subunit RPB2 antibody, polymerase (RNA) II (DNA directed) polypeptide B antibody, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog antibody, POLR2B antibody, polr2b antibody, LOC100640211 antibody, Polr2b antibody, polr2b.L antibody, rpb-2 antibody
- Background
-
POLR2B is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
Alias Symbols: RPB2, POL2RB, hsRPB2, hRPB140
Protein Interaction Partner: ESR1, UBC, TCEB3, SRC, PSMB9, SUMO2, XRN2, WWOX, SUZ12, RNF2, NEDD4, ITCH, RABEP2, HECW2, FBXO6, CDK20, HDAC11, ILK, POLR2C, MED19, MED26, RECQL5, VCP, BARD1, MDC1, POLR3F, TUBB4B, TUBA4A, POLR2I, POLR2E, POLR2D, POLR2A, DCTN1, ATP6V1B2, MEN1, CDK7, CCNH,
Protein Size: 1174 - Molecular Weight
- 134 kDa
- Gene ID
- 5431
- NCBI Accession
- NM_000938, NP_000929
- UniProt
- P30876
- Pathways
- Regulatory RNA Pathways, DNA Damage Repair
-