POLK antibody (N-Term)
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- Target See all POLK Antibodies
- POLK (Polymerase (DNA Directed) kappa (POLK))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Horse, Pig, Cow, Dog, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLK antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- KINKIIMEAT KGSRFYGNEL KKEKQVNQRI ENMMQQKAQI TSQQLRKAQL
- Predicted Reactivity
- Cow: 86%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%
- Characteristics
- This is a rabbit polyclonal antibody against POLK. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human POLK
- Top Product
- Discover our top product POLK Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 870 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- POLK (Polymerase (DNA Directed) kappa (POLK))
- Alternative Name
- POLK (POLK Products)
- Synonyms
- DINB1 antibody, DINP antibody, POLQ antibody, Dinb1 antibody, POLK antibody, si:ch211-254o18.3 antibody, DNA polymerase kappa antibody, polymerase (DNA directed) kappa antibody, polymerase (DNA directed), kappa antibody, polymerase (DNA directed) kappa L homeolog antibody, POLK antibody, Polk antibody, polk antibody, Tb11.01.0080 antibody, Tb11.01.0010 antibody, Tb11.01.0020 antibody, Tb11.01.0030 antibody, Tb11.01.0040 antibody, Tb11.01.0050 antibody, Tb11.12.0001 antibody, Tb11.12.0002 antibody, Tb11.12.0003 antibody, polk.L antibody, polk-1 antibody
- Background
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POLK belongs to the DNA polymerase type-Y family. It contains 2 Rad18-type zinc fingers and 1 umuC domain. POLK is a DNA polymerase specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. It lacks 3'-5' proofreading exonuclease activity. POLK forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Alias Symbols: DINB1, DINP, POLQ
Protein Interaction Partner: XPO7, MSH2, MCM6, MCM4, MSH6, TRAIP, UBC, REV1, Shprh, RAD18, PCNA, XRCC1, RPA1, POLD1,
Protein Size: 870 - Molecular Weight
- 99 kDa
- Gene ID
- 51426
- NCBI Accession
- NM_016218, NP_057302
- UniProt
- Q9UBT6
- Pathways
- DNA Damage Repair
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