Mediator Complex Subunit 25 (MED25) (N-Term) antibody

Details for Product No. ABIN2784291, Supplier: Log in to see
Antigen
  • Arc92
  • CG12254
  • Dmel\\CG12254
  • LD07688
  • Med25
  • dARC92
  • dDrip97
  • med25
  • ACID1
  • ARC92
  • CMT2B2
  • P78
  • PTOV2
  • RGD1305137
  • 2610034E13Rik
  • 2610529E18Rik
  • ESTM2
  • X83308
  • zgc:63555
  • Mediator complex subunit 25
  • mediator complex subunit 25
  • mediator of RNA polymerase II transcription, subunit 25 homolog (yeast)
  • MED25
  • med25
  • LOC100343629
  • Med25
Alternatives
anti-Human Mediator Complex Subunit 25 antibody for Western Blotting
Epitope
N-Term
11
10
9
3
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish
24
16
3
3
3
3
2
1
1
1
1
1
1
1
Host
Rabbit
24
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
23
14
14
4
1
1
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human MED25
Blocking Peptide Blocking peptide for this product available: ABIN979947
Sequence EGLRKHYLLP AIEYFNGGPP AETDFGGDYG GTQYSLVVFN TVDCAPESYV
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 85%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against MED25. It was validated on Western Blot.
Purification Affinity Purified
Alternative Name MED25 (MED25 Antibody Abstract)
Background This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
Alias Symbols: ACID1, ARC92, CMT2B2, DKFZp434K0512, MGC70671, P78, PTOV2, TCBAP0758
Protein Interaction Partner: UBC, MED19, MED26, EPAS1, NOTCH1, DREB2A, CTDP1, MED6, THRA, RXRA, RARA, MED1, NR3C1, ESR1, CREBBP, SREBF1, MED25, MED15, MED16, MED13, MED12, MED7, MED17, MED23, MED14, CDK8, ZC3H13, QKI, TRIP4, TADA2A, MED28, OBFC1, TRRAP, UL48, MED9, MED8, HCFC1, MED10
Protein Size: 747
Molecular Weight 78 kDa
Gene ID 81857
NCBI Accession NM_030973, NP_112235
UniProt Q71SY5
Pathways Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 747 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Mediator Complex Subunit 25 (MED25) (N-Term) antibody (ABIN2784291) WB Suggested Anti-MED25 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-Mediator Complex Subunit 25 (MED25) (N-Term) antibody (ABIN2784291) WB Suggested Anti-MED25
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 ...
Chromatin Immunoprecipitation (ChIP) image for anti-Mediator Complex Subunit 25 (MED25) (N-Term) antibody (ABIN2784291) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...
Product cited in: Sela, Conkright, Chen, Gilmore, Washburn, Florens, Conaway, Conaway: "Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6?." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 26179-87, 2013 (PubMed).