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ARHGEF4 antibody (N-Term)

ARHGEF4 Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Guinea Pig WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2787993
  • Target See all ARHGEF4 Antibodies
    ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))
    Binding Specificity
    • 8
    • 7
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Guinea Pig
    Host
    • 25
    • 5
    • 1
    Rabbit
    Clonality
    • 27
    • 4
    Polyclonal
    Conjugate
    • 11
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ARHGEF4 antibody is un-conjugated
    Application
    • 23
    • 17
    • 4
    • 1
    Western Blotting (WB)
    Sequence
    ADDYEAPRAG AREADDSGPE AQSCKDQMRT NVINEILSTE RDYIKHLRDI
    Predicted Reactivity
    Cow: 79%, Dog: 93%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%
    Characteristics
    This is a rabbit polyclonal antibody against Arhgef4. It was validated on Western Blot.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N-terminal region of Mouse Arhgef4
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 484 AA

    Restrictions
    For Research Use only
  • Validation #100106 (Western Blotting)
    'Independent Validation' Badge
    by
    Instituto de Parasitología y Biomedicina López-Neyra
    No.
    #100106
    Date
    12/23/2016
    Antigen
    ARHGEF4
    Lot Number
    QC30159-41213
    Method validated
    Western Blotting
    Positive Control
    primary mouse microglia
    Negative Control
    Notes
    Passed. ABIN2787993 specifically recognizes Arhgef4 in primary mouse microglia lysates.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN2787993
    Secondary Antibody
    anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
    Full Protocol
    • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
    • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma)).
    • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
    • Denature 5µg, 10µg and 30µg total protein for 5min at 95°C in 5µl, 10µl and 20µl Laemmli SDS sample buffer and subsequently separate them on a denaturing self-made 10% SDS-PAGE for 1h at 140V.
    • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
    • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk for 1h at RT.
    • Wash membrane 3x 5min with TBST.
    • Incubation with primary rabbit anti-ARHGEF4 antibody (antibodies-online, ABIN2787993, lot QC30159-41213) diluted 1:500 in TBST containing 2% BSA ON at 4°C.
    • Wash membrane 5x 5min with TBST.
    • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
    • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
    • Reveal protein bands using ECL Plus Western Blotting Detection Reagent (GE Healthcare, RPN2132, lot 4645641) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
    Experimental Notes
    The ARHGEF4 antibody ABIN2787993 reveals multiple bands as is expected for murine Arhgef4.
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))
    Alternative Name
    Arhgef4 (ARHGEF4 Products)
    Synonyms
    ARHGEF4 antibody, ASEF antibody, ASEF1 antibody, GEF4 antibody, STM6 antibody, 9330140K16Rik antibody, Asef antibody, ENSMUSG00000070955 antibody, Rho guanine nucleotide exchange factor 4 antibody, uncharacterized LOC100470646 antibody, rho guanine nucleotide exchange factor 4 antibody, Rho guanine nucleotide exchange factor (GEF) 4 antibody, ARHGEF4 antibody, arhg4 antibody, LOC100470646 antibody, LOC100544917 antibody, Arhgef4 antibody
    Background
    Arhgef4 acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion By similarity. It is required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Arhgef4 is involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
    Alias Symbols: 9330140K16Rik, Asef, ENSMUSG00000070955
    Protein Size: 484
    Molecular Weight
    57 kDa
    Gene ID
    226970
    NCBI Accession
    NM_183019, NP_898840
    UniProt
    Q7TNR9
    Pathways
    Neurotrophin Signaling Pathway
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