BAX antibody (BCL2-Associated X Protein) (N-Term)

Details for Product anti-BAX Antibody No. ABIN2792125, Supplier: Log in to see
Antigen
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • bax-A
  • xBax
  • xlbax
  • BAX
  • BCL2L4
  • BAX-ALPHA
  • bcl2-associated X protein, a
  • BCL2-associated X protein
  • Bcl2-associated X protein
  • baxa
  • bax
  • BAX
  • Bax
  • LOC100355675
Alternatives
anti-Human BAX antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
N-Term
105
65
31
28
21
20
15
15
13
12
11
10
10
10
8
8
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Pig (Porcine)
511
223
203
89
46
34
29
28
22
9
8
8
5
3
3
2
1
1
1
1
Host
Rabbit
333
208
4
Clonality
Polyclonal
Conjugate
This BAX antibody is un-conjugated
19
14
13
11
8
7
7
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
392
214
146
144
123
122
101
48
40
37
13
9
5
5
4
2
1
1
1
1
1
1
Options
Supplier
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human BAX
Sequence MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV
Predicted Reactivity Cow: 100%, Dog: 100%, Human: 100%, Pig: 100%
Characteristics This is a rabbit polyclonal antibody against BAX. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name BAX (BAX Antibody Abstract)
Background BAX encodes a protein that belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. BAX expression is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Alias Symbols: BCL2L4
Protein Interaction Partner: CYCS, ZBTB24, UBC, CASP9, CASP8, CASP7, CASP3, BCL2L1, BCL2, CLU, MDM2, MCL1, BAX, BAG3, YWHAB, VCP, CRYAB, CRYAA, BAK1, XIAP, TP53, KCNA3, HSPA4, XRCC6, XRCC5, HDAC6, BAD, PSMC5, PSMC3, PARK7, UHRF2, RNF144B, OYE2, TOMM22, TOMM40, MEPCE, MOAP1, BBC3, PPP
Protein Size: 192
Molecular Weight 21 kDa
Gene ID 581
NCBI Accession NM_138761, NP_620116
UniProt Q07812
Research Area Apoptosis/Necrosis
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded protein response
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 192 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-BCL2-Associated X Protein (BAX) (N-Term) antibody (ABIN2792125) WB Suggested Anti-BAX Antibody Titration: 1.25ug/ml
Positive Control:
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2-Associated X Protein (BAX) (N-Term) antibody (ABIN2792125) Rabbit Anti-BAX Antibody ,Paraffin Embedded Tissue: Human cardiac cell
Cellula...
Western Blotting (WB) image for anti-BCL2-Associated X Protein (BAX) (N-Term) antibody (ABIN2792125) Lanes: 1. Human NT-2 cells (641 µg) lysate 2. Mouse WT brain extract (841 µg)
P...
Product cited in: De Martino, Marfé, Longo, Fiorito, Montagnaro, Iovane, Decaro, Pagnini: "Bid cleavage, cytochrome c release and caspase activation in canine coronavirus-induced apoptosis." in: Veterinary microbiology, Vol. 141, Issue 1-2, pp. 36-45, 2010 (PubMed).

Honda, Abe, Hiroki, Honda, Iwanuma, Yanagisawa, Ide: "Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis." in: Journal of muscle research and cell motility, Vol. 28, Issue 4-5, pp. 243-7, 2007 (PubMed).

Background publications Yamaguchi, Chen, Bhalla, Wang: "Regulation of Bax activation and apoptotic response to microtubule-damaging agents by p53 transcription-dependent and -independent pathways." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 39431-7, 2004 (PubMed).

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