Cyclin A antibody (Cyclin A2) (Biotin)

Details for Product anti-CCNA2 Antibody No. ABIN2853583, Supplier: Log in to see
Antigen
  • ccn1
  • ccna
  • ccna1
  • CCN1
  • CCNA
  • AA408589
  • Ccn-1
  • Ccn1
  • Ccna
  • CycA2
  • Cyca
  • cb671
  • chunp6924
  • wu:fi36f03
  • CYCA
  • Cyclin-A2
  • ccna2
  • Cyclin-A
  • cyclinA
  • cyclin A2
  • cyclin A
  • ccna2
  • CCNA2
  • LOC100352785
  • Ccna2
  • LOC397933
Alternatives
anti-Human Cyclin A antibody for ELISA
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This Cyclin A antibody is conjugated to Biotin
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Application
Immunoprecipitation (IP), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen Bovine recombinant Cyclin A.
Clone E67-1
Isotype IgG2a
Specificity This antibody reacts with Cyclin A (55 kDa).
Purification Protein-A Agarose Chromatography of hybridoma supernatant.
Alternative Name Cyclin A2 (CCNA2 Antibody Abstract)
Background Cyclin A is a 60 kDa cyclin that binds both cdk2 and p34cdc2 (cdk1), giving two distinct cyclin A kinase activities, one appearing in G1/S phase, the other in G2/M. The level of cyclin A protein peaks in S-phase, and the activity of the cdk2-cyclin A complexes is essential for DNA replication and progression through S phase. Cyclin A is thus a marker for actively proliferating cells and for cells in S-phase. In normal cells, cdk2-cyclin A complexes associate with PCNA and p21CIPP1/WAF1. In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcrIPPtion factors DRT F1 and E2F, and retinoblastoma protein, p110.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2
Gene ID 890
NCBI Accession NP_001228
UniProt P20248
Research Area Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 20 µg/mL
Buffer PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Cyclin A2 (CCNA2) antibody (Biotin) (ABIN2853583) anti-Cyclin A2 (CCNA2) antibody (Biotin)
Background publications Di Bartolo, Cannon, Liu, Renne, Chadburn, Boshoff, Cesarman: "KSHV LANA inhibits TGF-beta signaling through epigenetic silencing of the TGF-beta type II receptor." in: Blood, Vol. 111, Issue 9, pp. 4731-40, 2008 (PubMed).

Ciarallo, Subramaniam, Hung, Lee, Kotchetkov, Sandhu, Milic, Slingerland: "Altered p27(Kip1) phosphorylation, localization, and function in human epithelial cells resistant to transforming growth factor beta-mediated G(1) arrest." in: Molecular and cellular biology, Vol. 22, Issue 9, pp. 2993-3002, 2002 (PubMed).

Sandhu, Donovan, Bhattacharya, Stampfer, Worland, Slingerland: "Reduction of Cdc25A contributes to cyclin E1-Cdk2 inhibition at senescence in human mammary epithelial cells." in: Oncogene, Vol. 19, Issue 47, pp. 5314-23, 2000 (PubMed).

Sandhu, Peehl, Slingerland: "p16INK4A mediates cyclin dependent kinase 4 and 6 inhibition in senescent prostatic epithelial cells." in: Cancer research, Vol. 60, Issue 10, pp. 2616-22, 2000 (PubMed).

Slingerland, Hengst, Pan, Alexander, Stampfer, Reed: "A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells." in: Molecular and cellular biology, Vol. 14, Issue 6, pp. 3683-94, 1994 (PubMed).

Dveksler, Dieffenbach, Cardellichio, McCuaig, Pensiero, Jiang, Beauchemin, Holmes: "Several members of the mouse carcinoembryonic antigen-related glycoprotein family are functional receptors for the coronavirus mouse hepatitis virus-A59." in: Journal of virology, Vol. 67, Issue 1, pp. 1-8, 1993 (PubMed).

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