GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN285794, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
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Host
Rabbit
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2
Clonality
Polyclonal
Conjugate
This GFP antibody is un-conjugated
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Application
Immunoprecipitation (IP), Western Blotting (WB)
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Immunogen GFP antibody was raised in rabbit using E. Coli-expressed full length green fluorescent protein as the immunogen.
Specificity This antibody neutralizes and binds to human interferon alpha receptor with high affinity. It interacts with extracellular domain and blocks biological action of Type I interferons.
Cross-Reactivity (Details) This antibody reacts with wild -type GFP and it's variants, such as EGFP, EBFP
Purification purified
Alternative Name GFP
Target Type Tag
Background The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
Research Area Tags/Labels
Application Notes IP: 1:200-1:500, WB: 1:5,000
Optimal conditions should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitue in distilled water.
Concentration Lot specific
Buffer Supplied as a lyophilized Potein A purified rabbit IgG with 0.1 % NaN3
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Storage RT/-20 °C
Storage Comment Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Supplier Images
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN285794) Lane 1 - Marker; Lane - GP fusion protein; Lane 3 - Control
Product cited in: Orozco, Tahirovic, Rentzsch, Schwenk, Haass, Edbauer: "Loss of fused in sarcoma (FUS) promotes pathological Tau splicing." in: EMBO reports, Vol. 13, Issue 8, pp. 759-64, 2012 (PubMed).