Collagen Type I antibody

Catalog No. ABIN289402

This Rabbit Polyclonal antibody specifically detects Collagen Type I in WB, IHC and ELISA. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for Collagen Type I antibody (ABIN289402)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Collagen Type I Antibody

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Less than 0.1% crossreactivity with Type II, III, IV, V or VI Collagens.

Characteristics: Affinity purified Rabbit polyclonal Collagen Type I antibody
Bioactivity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues

Purification: Affinity chromatography purified

Sterility: Filtered

Immunogen: Collagen type I antibody was raised in rabbit using collagen type I from human and bovine placenta as the immunogen.

Application Details

Application Notes: ELISA: 1:5,000-1:50,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:500-1:5,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered liquid in 0.125 M Na5 5 7, pH 8.0, with 0.075 NaCl, 0.005 EDTA, and 0.01 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

References for anti-Collagen Type I antibody (ABIN289402)

Okorie, Diamond: "Matrix protein microarrays for spatially and compositionally controlled microspot thrombosis under laminar flow." in: Biophysical journal, Vol. 91, Issue 9, pp. 3474-81, (2006) (PubMed).

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I

Background: Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Type-I collagen is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone.